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	Provedor de dados OAK (1) Autor Fujisaki, Kozo (1) Jia, Honglin (1) Nishikawa, Yoshifumi (1) Xuan, Xuenan (1) Zhou, Jinlin (1) Palavra-chave Babesia gibsoni (1) Diagnosis (1) Enzyme-linked immunosorbent assay (1) Rhoptry-associated protein (1) Ano 2007 (1) País Japan (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen OAK Zhou, Jinlin; Jia, Honglin; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan. A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or... Palavras-chave: Babesia gibsoni; Rhoptry-associated protein; Enzyme-linked immunosorbent assay; Diagnosis. Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/811 Registros recuperados: 1 Primeira ... 1 ... Última

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